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BRD4 (BD1/BD2)

Bromodomain-containing protein 4, HUNK1

Catalog Number:
E2208T-H09H
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Description:Recombinant human BRD4 (BD1/BD2) protein with N-terminal 6x His tag was purified by Ni-NTA affinity and followed by SEC chromatography. The BRD4 (BD1/BD2) protein showed high activity in HTRF assay.


Species:Human


Tag:N-terminal 6xHis tag


Expression Host:HEP E.coli


Uniprot: O60885-1


Sequences:E49-E460 (end)


Molecular Weight:48.6 kDa


Formulation:50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5


Form:Liquid


Purity:>95%


Stability & Storage:Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.


SDS-PAGE:

  BRD4(BD1&BD2)-1.png


Activity Identification:The BRD4 (BD1/BD2) activity was assayed using HTRF technology. The reaction was performed by incubating the BRD4 (BD1-BD2) protein , BET and the beads at 25℃ for 60 min, then reading ratio 665/620 nm with BMG.

BRD4(BD1-BD2)-2.pngBRD4(BD1&BD2)-3.png

Note:

1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.

2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.

3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.

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