METTL3/METTL14
N6-adenosine-methyltransferase catalytic subunit, MTA70
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Catalog Number:S2206F-H01H2
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Description：Recombinant human METTL3 protein with N-terminal Flag tag was co-expressed with recombinant human METTL14 with N-terminal 6x His tag. Recombinant human METTL3/METTL14 protein was purified by Ni-NTA affinity and followed by SEC chromatography. The METTL3/METTL14 protein showed high activity in HTRF assay.
Species：Human
Tag：N-terminal Flag tag
Expression Host：HEP sf9
Uniprot： METTL3: Q86U44-1; METTL14: Q9HCE5
Sequences： METTL3: S2-L580(end); METTL14: D2-R456(end)
Molecular Weight： METTL3: 66.6 kDa; METTL14: 54.1 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>95%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The METTL3/METTL14 activity was detected using HTRF technology. The reaction was performed by incubating the METTL3/METTL14 protein and Bio-METTL3/ METTL14-RNA&SAM at 25℃ for 60 min, adding the YTHDF1 and beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
