Histone H2A
Histone H2A type 1-C, H2AFL, HIST1H2AC, H2AC6
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Catalog Number:E2204F-H12H
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Size：
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human Histone H2A protein with N -terminal 6x His tag was purified was denatured, and then purified by Ni-NTA affinity. After renaturation, it was further separated by SEC chromatography. The Histone H2A protein showed high activity in MTase-Glo assay.
Species：Human
Tag：N-terminal 6xHis tag
Expression Host：HEP E.coli
Uniprot： Q93077
Sequences：M1-K130(end)
Molecular Weight：16.2 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Histone H2A activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the PRMT5 protein, SAM and Histone H2A at 25℃ for 180 min, then adding MTase-Glo reagent and reading RLU signal with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
