DOT1L
Histone-lysine N-methyltransferase, H3 lysine-79 specific, KMT4
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Catalog Number:E2303T-H12G
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human DOT1L protein with N-terminal GST tag was purified by GST affinity and followed by SEC chromatography. The DOT1L protein showed high activity in MTase-Glo assay.
Species：Human
Tag：N-terminal GST tag
Expression Host：HEP E.coli
Uniprot：Q8TEK3-2
Sequences：M1-K416
Molecular Weight：73.7 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>95%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The DOT1L activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the DOT1L protein, SAM and substrate at 25℃ for 180 min, then adding MTase-Glo reagent and reading RFU with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
