CRBN/DDB1
Protein cereblon
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Catalog Number:S2408F-M01H2
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Size：
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COA:Please contact us
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Price:Please contact us
Description：Recombinant mouse CRBN with N-terminal 8x His tag was co-expressed with recombinant mouse DDB1 protein. Recombinant mouse CRBN/DDB1 protein with N-terminal 8x His tag was purified by Ni-NTA affinity and followed by desalting. The CRBN/DDB1 protein showed high activity in TR-FRET assay.
Species：Mouse
Tag：CRBN: N-terminal 8x His tag; DDB1: no tag
Expression Host：HEP sf9
Uniprot：CRBN: Q8C7D2-1; DDB1: Q3U1J4
Sequences：CRBN: M1-L445(end); DDB1: M1-H1140(end)
Molecular Weight：CRBN: 53.3 kDa; DDB1: 126.9 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>70%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The CRBN/DDB1 activity was detected using TR-FRET technology. The reaction was performed by incubating the CRBN/DDB1 protein and Thalidomide-BODIPY, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 520/490 with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
