Biotin-CUL3/RBX2
Cullin-3
-
Catalog Number:S2407F-H08HA2
-
Size：
-
COA:Please contact us
-
Price:Please contact us
Description：Recombinant human CUL3 with N-terminal 6x His and Avi tag was co-expressed with recombinant human RBX2 protein and performed in vivo biotinylation simultaneously. Recombinant human Biotin-CUL3/RBX2 protein was purified by Ni-NTA affinity and followed by SEC chromatography. The Biotin-CUL3/RBX2 protein showed good activity in HTRF assay.
Species：Human
Tag：CUL3: N-terminal 6x His and Avi tag；RBX2: no tag
Expression Host：HEP sf9
Uniprot：CUL3: Q13618-1; RBX2: Q9UBF6-1
Sequences：CUL3: M1-A768(end); RBX2: M1-K113(end)
Molecular Weight：CUL3: 92.9 kDa; RBX2: 12.7 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-CUL3/RBX2 activity was detected using HTRF technology. The reaction was performed by incubating the Biotin-CUL3/RBX2 protein and KEAP1 protein, and adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
