推荐 CBL-B
E3 ubiquitin-protein ligase CBL-B, RNF56
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Catalog Number:E2203T-H10HA
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Description：Recombinant human CBL-B protein with N-terminal 6x His tag and Avi tag was purified by Ni-NTA affinity and followed by SEC chromatography, The CBL-B protein showed high activity in TR-FRET assay.
Species：Human
Tag： N-terminal His&Avi tag
Expression Host：HEP E.coli
Uniprot： Q13191
Sequences：A39-F426
Molecular Weight：47.9 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>95%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The CBL-B activity was detected using TR-FRET technology. The reaction was performed by incubating the CBL-B protein, tracer and adding the beads and keeping the mixture at 25℃ for 60 min, then reading the ratio 520/490 with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
