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USP1/UAF1

Ubiquitin carboxyl-terminal hydrolase 1

Catalog Number:
S2310F-H22H2
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Description:Recombinant human USP1 with C-terminal 8x His tag was co-expressed with UAF1 protein with C-terminal 8x His tag. Recombinant human USP1/UAF1 protein was purified by Ni-NTA affinity and followed by desalting. The USP1/UAF1 protein showed high activity in FI assay.


Species:Human


Tag:USP1: C-terminal His tag; UAF1: C-terminal His tag


Expression Host:HEP sf9


Uniprot:USP1: O94782; UAF1: Q8TAF3


Sequences:USP1: M1-L785(end); UAF1: M1-T677(end)


Molecular Weight:USP1: 89.4 kDa; UAF1: 77.5 kDa


Formulation:50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5


Form:Liquid


Purity:>80%


Stability & Storage:Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.


SDS-PAGE:

  S2310F-H22H2-gel.png


Activity Identification:The USP1/UAF1 activity was detected by FI assay. The reaction was performed by incubating the USP1/UAF1 protein and substrate at 25℃ for 30 min, then reading RFU with BMG.  

S2310F-H22H2-1.pngS2310F-H22H2-2.png

Note:

1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.

2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.

3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.

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