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COP1(WD40)

E3 ubiquitin-protein ligase COP1, RFWD2

Catalog Number:
S2307T-H15H
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Description:Recombinant human COP1(WD40) protein with N-terminal 6x His tag was purified by Ni-NTA affinity and followed by SEC chromatography. The COP1(WD40) protein showed good activity in TR-FRET assay.


Species:Human


Tag:N-terminal His tag


Expression Host:HEP sf9


Uniprot: Q8NHY2


Sequences:A386-V731 (end)


Molecular Weight:40 kDa


Formulation:50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5


Form:Liquid


Purity:>95%


Stability & Storage:Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.


SDS-PAGE:

  S2307T-H15H-GEL.png


Activity Identification:The COP1(WD40) activity was detected using TR-FRET technology. The reaction was performed by incubating the COP1(WD40) protein, TRIB1 and the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG. 

S2307T-H15H-1.pngS2307T-H15H-2.png

Note:

1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.

2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.

3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.

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