Biotin-CRBN
Protein cereblon
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Catalog Number:E2309T-H26H
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Size：
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human Biotin-CRBN protein with N-terminal 6x His & BAP tag and C-terminal Avi tag was purified by Ni-NTA affinity and followed by SEC chromatography. The Biotin-CRBN protein showed high activity in SPR binding assay with small molecular.
Species：Human
Tag： N-terminal His-BAP tag, C-terminal Avi tag
Expression Host：HEP E.coli
Uniprot： Q96SW2-1
Sequences：T319-L442(end)
Molecular Weight：18.8 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-CRBN activity was detected using SPR technology. The Biotin-CRBN protein was immobilized onto the SA chip. The varying concentration of Eragidomide was injected onto the Biotin-CRBN protein surface, and recorded the response signal. The kinetics fitting was as follows with a kD value of ~ 2.94x10-6 M.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
