Biotin-c-Cbl
E3 ubiquitin-protein ligase CBL, CBL2, RNF55
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Catalog Number:E2306T-H09HA
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human Biotin-c-Cbl protein with N-terminal 6x His tag and Avi tag was purified by Ni-NTA affinity, and followed by biotinylation on Avi tag and SEC chromatography. The Biotin-c-Cbl protein showed high activity in SPR binding assay with small molecular.
Species：Human
Tag：N-terminal His tag-Avi tag
Expression Host：HEP E.coli
Uniprot： P22681
Sequences：G49-F434
Molecular Weight：48.6 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>95%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-c-Cbl activity was detected using SPR technology. The Biotin-c-Cbl protein was immobilized onto the SA chip using labeled biotin. The varying concentration of small molecular was injected onto the Biotin-c-Cbl protein surface, and recorded the response signal. The kinetics fitting was as follows with a kD value of ~5x10-8 M.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
