VHL/Elongin-C/Elongin-B Protein
VCB
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Catalog Number:E2204F-H16H3
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human VHL/Elongin-C/Elongin-B(VCB) protein with N-terminal 8x His tag was purified by Ni-NTA affinity and followed by SEC chromatography.
Species：Human
Tag：N-terminal His tag
Expression Host：HEP E.coli
Uniprot： VHL: P40337/Elong C: Q15369/Elong B: Q15370
Sequences：VHL: M54-D213 (end)/ Elong C: M17-C112(end)/ Elong B: M1-K104
Molecular Weight：VHL: 21.0 kDa; Elong C: 10.8 kDa; Elong B: 11.7 kDa
Formulation：50 mM Tris, 200 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
Form：Liquid
Purity：>90%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The VCB activity was detected using SPR technology. The VCB protein was immobilized onto the CM5 chip. The varying concentration of small molecular was injected onto the VCB protein surface, and recorded the response signal. The kinetics fitting was as follows with a kD value of ~ 3.98x10-8 M.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
